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Home >> Antibodies >> GFAP Antibody / Glial Fibrillary Acidic Protein

GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/8616R] (V4513)

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  Catalog No Formulation Size Price (USD)  
Image V4513-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4513-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4513SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/8616R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/8616R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/8616R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GFAP/8616R
Purity Protein A/G affinity
UniProt P14136
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This GFAP antibody is available for research use only.
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Description

GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the GFAP antibody.

Storage

Aliquot the GFAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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