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Home >> Antibodies >> GFAP Antibody / Glial Fibrillary Acidic Protein

GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/6884] (V4004)

  Catalog No Formulation Size Price (USD)  
Image V4004-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4004-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4004SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Western blot testing of human brain tissue lysate using GFAP antibody (clone GFAP/6884). Predicted molecular weight ~50 kDa.
IHC staining of FFPE human cerebellum tissue with GFAP antibody (clone GFAP/6884). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/6884). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GFAP antibody (clone GFAP/6884). These results demonstrate the foremost specificity of the GFAP/6884 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name GFAP/6884
Purity Protein A/G affinity
UniProt P14136
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This GFAP antibody is available for research use only.
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Description

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.

Immunogen

A portion of amino acids 150-250 from the human GFAP protein was used as the immunogen for the GFAP antibody.

Storage

Aliquot the GFAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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