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Home >> Antibodies >> GFAP Antibody Cocktail

GFAP Antibody Cocktail [clone GA-5 + ASTRO/789] (V2529)

  Catalog No Formulation Size Price (USD)  
Image V2529-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2529-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2529SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2529IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP antibody cocktail (GA-5 + ASTRO/789).
IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with GFAP antibody cocktail (GA-5 + ASTRO/789).
IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody cocktail (GA-5 + ASTRO/789).
Western blot testing of human brain lysate with GFAP antibody cocktail (clones GA-5 + ASTRO/789). Predicted molecular weight ~50 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name GA-5 + ASTRO/789
Purity Protein G affinity chromatography
UniProt P14136
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This GFAP antibody cocktail is available for research use only.
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Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

Optimal dilution of the GFAP antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

GFAP isolated from pig spinal cord (GA-5) and recombinant protein (ASTRO/789) were used as the immunogen for the GFAP antibody cocktail.

Storage

Store the GFAP antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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