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Home >> Antibodies >> GDP-L-fucose synthase Antibody / GFUS / TSTA3

GDP-L-fucose synthase Antibody / GFUS / TSTA3 (RQ6910)

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  Catalog No Formulation Size Price (USD)  
Image RQ6910 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human MCF7 cells with GDP-L-fucose synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with GDP-L-fucose synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human MCF7, 2) human HeLa, 3) human HepG2, 4) human RT-4, 5) human PC-3, 6) rat testis, 7) rat kidney, 8) rat brain, 9) mouse testis and 10) mouse kidney tissue lysate with GDP-L-fucose synthase antibody. Predicted molecular weight ~36 kDa.
Flow cytometry testing of human U-87 MG cells with GDP-L-fucose synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GDP-L-fucose synthase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13630
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This GDP-L-fucose synthase antibody is available for research use only.
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Description

Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.

Application Notes

Optimal dilution of the GDP-L-fucose synthase antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K44-K321) was used as the immunogen for the GDP-L-fucose synthase antibody.

Storage

After reconstitution, the GDP-L-fucose synthase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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