GCSF Antibody / CSF3 Cytokine Signaling Marker [clone CSF3/4594] (V4471)

GCSF Antibody U-87 WB. Western blot analysis of Granulocyte colony stimulating factor / CSF3 in human U-87 cell lysate using mouse monoclonal GCSF antibody, clone CSF3/4594. A band is detected at approximately 22 kDa, consistent with the predicted molecular weight of G-CSF, supporting detection of this cytokine in glioblastoma-derived cells.

GCSF Antibody Renal Tissue IHC. Immunohistochemistry of Granulocyte colony stimulating factor / CSF3 in FFPE human kidney tissue using mouse monoclonal GCSF antibody, clone CSF3/4594. HRP-DAB brown staining highlights cytoplasmic labeling of renal tubular epithelial cells, consistent with cytokine-associated expression in metabolically active tissue, while surrounding interstitial cells show lower staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free GCSF antibody (clone CSF3/4594) as confirmation of integrity and purity.

GCSF Antibody Microarray Specificity Validation. Protein microarray analysis of Granulocyte colony stimulating factor / CSF3 using mouse monoclonal GCSF antibody, clone CSF3/4594, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CSF3 with minimal off-target interaction. The signal profile shows strong enrichment for CSF3 relative to other proteins on the array, supporting high specificity in a proteome-wide context. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, confirming selective recognition of GCSF.