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Home >> Antibodies >> GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit [clone 28G35] (RQ8371)

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Image RQ8371 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
Microvalidated Recrabbitmono
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IHC staining of FFPE mouse adrenal tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse adrenal tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human clear cell renal cell carcinoma tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid papillary carcinoma tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with GCLM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human A549, 3) human Jurkat, 4) human K562, 5) rat liver and 6) mouse liver tissue lysate with GCLM antibody. Predicted molecular weight: 28-31 kDa (two isoforms).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name 28G35
Purity Affinity chromatography
UniProt P48507
Localization Cytoplasm
Applications Western Blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This GCLM antibody is available for research use only.
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Description

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit.

Application Notes

Optimal dilution of the GCLM antibody should be determined by the researcher.

Immunogen

A synthetic peptide derived from human GCLM was used as the immunogen for the GCLM antibody.

Storage

Store the GCLM antibody at -20oC.

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