GCHFR Antibody / Tetrahydrobiopterin Metabolism Regulator Antibody (RQ6178)

GCHFR Antibody Tonsil IHC. Immunohistochemistry analysis of FFPE human tonsil tissue stained with GCHFR Antibody demonstrates diffuse cytoplasmic HRP-DAB brown staining throughout lymphoid-associated cellular populations, consistent with the role of GTP cyclohydrolase I feedback regulator / GCHFR in tetrahydrobiopterin metabolism and nitric oxide-associated cellular signaling pathways. The observed staining profile aligns with the involvement of BH4 regulatory pathways in immune-associated metabolic activity and cytokine-responsive signaling environments. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

GCHFR Antibody Rectal Cancer IHC. Immunohistochemistry analysis of FFPE human rectal cancer tissue stained with GCHFR Antibody demonstrates granular cytoplasmic HRP-DAB brown staining throughout gland-forming tumor epithelial cellular populations, consistent with expression of GTP cyclohydrolase I feedback regulator / GCHFR in tetrahydrobiopterin-associated metabolic signaling and nitric oxide regulatory pathways. The observed staining pattern aligns with the intracellular metabolic regulatory function of this BH4-associated feedback protein in proliferative epithelial tissue environments. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

GCHFR Antibody Prostate Cancer IHC. Immunohistochemistry analysis of FFPE human prostate cancer tissue stained with GCHFR Antibody demonstrates cytoplasmic HRP-DAB brown staining within glandular tumor epithelial cellular populations, consistent with the role of GTP cyclohydrolase I feedback regulator / GCHFR in tetrahydrobiopterin metabolism and nitric oxide-associated metabolic regulation pathways. The observed staining profile aligns with the intracellular cofactor regulatory biology of GCHFR within epithelial-associated tissue compartments. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

GCHFR Antibody Mouse Liver IHC. Immunohistochemistry analysis of FFPE mouse liver tissue stained with GCHFR Antibody demonstrates diffuse cytoplasmic HRP-DAB brown staining throughout hepatocyte-associated cellular populations, consistent with the role of GTP cyclohydrolase I feedback regulator / GCHFR in tetrahydrobiopterin metabolism and nitric oxide-associated metabolic signaling pathways. The observed hepatic staining profile aligns with the intracellular cofactor regulatory function of GCHFR in metabolic homeostasis and redox-associated cellular regulation. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

GCHFR Antibody Caco-2 IF. Immunofluorescence analysis of FFPE human Caco-2 cells stained with GCHFR Antibody demonstrates diffuse cytoplasmic green fluorescence consistent with the localization of GTP cyclohydrolase I feedback regulator / GCHFR, a BH4-associated metabolic regulatory protein involved in nitric oxide signaling and cofactor homeostasis pathways. DAPI nuclear counterstain is shown in blue. HIER: steam sections in pH 6 citrate buffer for 20 min prior to staining.

GCHFR Antibody Multi-Species WB. Western blot analysis of human HEK293, K562, HepG2, Caco-2, HeLa, U937, and PC-3 cell lysates together with rat liver and mouse liver tissue lysates using GCHFR Antibody demonstrates bands at approximately 12 kDa, consistent with the predicted molecular weight of GTP cyclohydrolase I feedback regulator / GCHFR. The observed cross-species expression profile aligns with the role of GCHFR as a tetrahydrobiopterin-associated metabolic regulatory protein involved in nitric oxide signaling, cofactor homeostasis, and cellular redox-associated pathways. Stronger signal intensity is observed in HepG2, U937, rat liver, and mouse liver lysates, consistent with metabolically active tissue-associated expression patterns.

GCHFR Antibody HL60 FACS. Flow cytometry analysis of HL60 cells stained with GCHFR Antibody demonstrates a clear rightward shift of the positive cell population relative to the isotype control, consistent with detection of GTP cyclohydrolase I feedback regulator / GCHFR expression in metabolically active hematopoietic-derived cells. Blue=GCHFR antibody, Green=isotype control, Red=cells alone control. The observed staining profile aligns with the role of GCHFR in tetrahydrobiopterin metabolism, nitric oxide-associated signaling, and intracellular cofactor regulatory pathways.