GBP1 Antibody / Interferon-Induced Immune Response Marker (FY12213)

GBP1 Antibody Ovarian Cancer IHC. Immunohistochemistry analysis of paraffin-embedded human ovarian cancer tissue stained with GBP1 antibody demonstrates moderate cytoplasmic HRP-DAB brown staining throughout malignant epithelial and stromal-associated cellular populations, consistent with interferon-responsive inflammatory signaling and innate immune activation pathways involving Guanylate binding protein 1 / GBP1. The observed staining pattern aligns with the role of GBP1 as an interferon-inducible large GTPase associated with host defense and immune-associated tissue responses. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0).

GBP1 Antibody Multi-Species WB. Western blot analysis of human placenta tissue lysate, human HUVEC whole cell lysate, human MCF-7 whole cell lysate, human HeLa whole cell lysate, rat brain tissue lysate, rat spleen tissue lysate, mouse brain tissue lysate, and mouse spleen tissue lysate using GBP1 antibody demonstrates a distinct band at approximately 68 kDa, consistent with the expected molecular weight of Guanylate binding protein 1 / GBP1. The observed cross-species expression profile supports the role of GBP1 as an interferon-induced GTPase involved in innate immune signaling, inflammatory activation, and host defense-associated pathways. Detection was performed using rabbit anti-GBP1 antibody followed by HRP-conjugated goat anti-rabbit secondary antibody and enhanced chemiluminescent development.

GBP1 Antibody Mouse Heart IHC. Immunohistochemistry analysis of paraffin-embedded mouse heart tissue stained with GBP1 antibody demonstrates cytoplasmic HRP-DAB brown staining within cardiomyocyte-associated and stromal cellular populations, consistent with interferon-responsive GBP1 expression in inflammatory and stress-associated tissue environments. The staining profile aligns with the role of Guanylate binding protein 1 / GBP1 as an innate immune-associated large GTPase involved in cytokine-responsive signaling pathways. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0).

GBP1 Antibody Rat Heart IHC. Immunohistochemistry analysis of paraffin-embedded rat heart tissue stained with GBP1 antibody demonstrates diffuse cytoplasmic HRP-DAB brown staining within cardiomyocyte-associated cellular populations, consistent with the role of Guanylate binding protein 1 / GBP1 in interferon-responsive inflammatory signaling and stress-associated innate immune pathways. The observed staining pattern supports the established function of GBP1 as an interferon-inducible host defense GTPase involved in immune-associated cellular activation. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0).

GBP1 Antibody SiHa IF. Immunofluorescence analysis of SiHa cells stained with GBP1 antibody demonstrates diffuse cytoplasmic green fluorescence consistent with the localization of Guanylate binding protein 1 / GBP1 as an interferon-inducible large GTPase involved in innate immune signaling and inflammatory response pathways. DAPI nuclear counterstain is shown in blue. Enzyme antigen retrieval was performed for 15 min prior to staining, followed by DyLight 488-conjugated goat anti-rabbit secondary antibody detection.

GBP1 Antibody HeLa IP-WB. Immunoprecipitation of GBP1 from HeLa whole cell lysate followed by western blot analysis using GBP1 antibody demonstrates a distinct band at approximately 68 kDa, consistent with the expected molecular weight of Guanylate binding protein 1 / GBP1. Lane 1: HeLa whole cell lysate input control (30 ug). Lane 2: rabbit IgG negative control immunoprecipitation. Lane 3: immunoprecipitation using anti-GBP1 antibody (2 ug) with HeLa whole cell lysate (500 ug). The selective enrichment observed in the GBP1 immunoprecipitation lane supports specific detection of this interferon-inducible large GTPase involved in innate immune signaling and inflammatory response pathways. Detection was performed using rabbit anti-GBP1 antibody followed by HRP-conjugated goat anti-rabbit secondary antibody and ECL substrate development.

GBP1 Antibody MCF-7 FACS. Flow cytometry analysis of MCF-7 cells stained with GBP1 antibody demonstrates a clear rightward shift of the positive cell population relative to the isotype control, consistent with detection of Guanylate binding protein 1 / GBP1 expression in epithelial-derived tumor cells. Blue=GBP1 antibody, Green=isotype control, Red=blank control. Cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and detected using DyLight 488-conjugated goat anti-rabbit secondary antibody.