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Home >> Antibodies >> GATA6 Antibody

GATA6 Antibody (RQ6769)

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  Catalog No Formulation Size Price (USD)  
Image RQ6769 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with GATA6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with GATA6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human Caco-2 cells with GATA6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T, 2) MCF7 and 3) PC-3 cell lysate with GATA6 antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of human U-2 OS cells with GATA6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GATA6 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q92908
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This GATA6 antibody is available for research use only.
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Description

Transcription factor GATA-6, also known as GATA-binding factor 6 (GATA6), is protein that in humans is encoded by the GATA6 gene. This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects.

Application Notes

Optimal dilution of the GATA6 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids N105-L151) was used as the immunogen for the GATA6 antibody.

Storage

After reconstitution, the GATA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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