GATA3 Antibody / Breast Lineage Marker Antibody [clone GATA3/2444] (V7805)

GATA3 Antibody Breast Carcinoma IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue stained with clone GATA3/2444 demonstrates strong nuclear HRP-DAB brown staining within malignant epithelial cells, consistent with the established role of GATA binding protein 3 / GATA3 as a breast lineage-associated transcription factor involved in luminal epithelial differentiation.

GATA3 Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using clone GATA3/2444 demonstrates highly selective binding to GATA binding protein 3 / GATA3 with minimal off-target reactivity detected across the tested human proteome. The strong enrichment of GATA3 signal relative to lower-ranking proteins supports the specificity of this monoclonal antibody for studies involving epithelial differentiation, breast lineage-associated transcriptional regulation, and T-cell lineage biology.
Z- and S-score interpretation: The Z-score represents the signal intensity generated when an antibody, together with a fluorescently labeled secondary antibody, binds to a specific protein target on the HuProt(TM) array. Z-scores are expressed as standard deviations above the mean signal intensity of all proteins present on the array. Proteins are ranked according to descending Z-score values, and the S-score represents the difference in Z-score between sequentially ranked proteins. The S-score therefore reflects the relative specificity of an antibody for its intended target compared to other proteins represented on the array.

SDS-PAGE analysis of purified, BSA-free GATA3 antibody as confirmation of integrity and purity.