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Home >> Antibodies >> GATA3 Antibody

GATA3 Antibody [clone GATA3/2444] (V7805)

  Catalog No Formulation Size Price (USD)  
Image V7805-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7805-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7805SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC staining of FFPE human breast carcinoma with GATA3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GATA3 antibody (clone GATA3/2444). These results demonstrate the foremost specificity of the GATA3/2444 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free GATA3 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name GATA3/2444
Purity Protein G affinity chromatography
UniProt P23771
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This GATA3 antibody is available for research use only.
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Description

GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas. Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Application Notes

Optimal dilution of the GATA3 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 357-436) was used as the immunogen for the GATA3 antibody.

Storage

Store the GATA3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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