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Home >> Antibodies >> GATA-3 Antibody

GATA-3 Antibody [clone GATA3/7685R] (V4083)

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  Catalog No Formulation Size Price (USD)  
Image V4083-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4083-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4083SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human bladder tissue with GATA-3 antibody (clone GATA3/7685R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human bladder tissue with GATA-3 antibody (clone GATA3/7685R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carinoma tissue with GATA-3 antibody (clone GATA3/7685R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma tissue with GATA-3 antibody (clone GATA3/7685R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GATA3/7685R
Purity Protein A/G affinity
UniProt P23771
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This GATA-3 antibody is available for research use only.
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Description

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Application Notes

Optimal dilution of the GATA-3 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein (within amino acids 150-250) from the human protein was used as the immunogen for the GATA-3 antibody.

Storage

Aliquot the GATA-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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