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Home >> Antibodies >> GAPDH Loading Control Antibody

GAPDH Loading Control Antibody (R34262)

  Catalog No Formulation Size Price (USD)  
Image R34262-100UG 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide 100 ug 429
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Western blot testing of human 1) HEK293 and 2) HeLa cell lysate with GAPDH loading control antibody at 0.1ug/ml. Predicted molecular weight ~36 kDa.
Western blot testing of human 1) liver and 2) tonsil lysate with GAPDH loading control antibody at 0.1ug/ml. Predicted molecular weight ~36 kDa.
Western blot testing of 1) mouse liver and 2) rat brain lysate with GAPDH loading control antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
IHC testing of FFPE human kidney tissue with GAPDH loading control antibody at 5ug/ml (inset without primary Ab). Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
IF/ICC staining of human HeLa cells with Alexa Fluor 488 secondary, and GAPDH antibody at 5ug/ml (blue = DAPI nuclear counterstain).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Pig
Predicted Reactivity Dog
Format Antigen affinity purified
Clonality Polyclonal (goat origin)
Isotype Goat Ig
Purity Antigen affinity
Gene ID 2597
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IF/ICC : 5ug/ml
IHC (FFPE) : 5ug/ml
ELISA (peptide) LOD : 1:128000
Limitations This GAPDH loading control antibody is available for research use only.
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Related Products

  • Applications : WB, IF/ICC ELISA (peptide)
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Dog, Pig

Description

Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase; GAPDH

Application Notes

Optimal dilution of the GAPDH loading control antibody should be determined by the researcher.

Immunogen

Amino acids HQVVSSDFNSDT were used as the immunogen for this GAPDH loading control antibody.

Storage

Aliquot and store the GAPDH loading control antibody at -20oC.

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