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Home >> Antibodies >> GAPDH Antibody

GAPDH Antibody [clone BH-7] (RQ5052)

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  Catalog No Formulation Size Price (USD)  
Image RQ5052 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of 1) human HeLa, 2) human Jurkat, 3) mouse kidney, 4) mouse spleen, 5) mouse RAW264 and 6) rat brain lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.
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Western blot testing of 1) human HeLa, 2) human Jurkat, 3) mouse kidney, 4) mouse spleen, 5) mouse RAW264 and 6) rat brain lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.
IF/ICC staining of human HeLa cells with GAPDH antibody (green) and DAPI nuclear stain (blue).
IHC staining of FFPE human uterus with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human kidney with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat colon with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name BH-7
Purity Affinity purified
UniProt P04406
Applications Western blot : 1:3000-1:10000
Immunohistochemistry (FFPE) : 1:100-1:500
Immunofluorescence/Immunocytochemistry : 1:100-1:250
Limitations This GAPDH antibody is available for research use only.
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Description

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). [RefSeq]

Application Notes

Optimal dilution of the GAPDH antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human GAPDH was used as the immunogen for the GAPDH antibody.

Storage

Store the GAPDH antibody at -20oC.

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