- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GAPDH Antibody (R32661)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R32661 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P04406 |
| Localization | Cytoplasmic, nuclear |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This GAPDH antibody is available for research use only. |
|
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GAPDH antibody is an important reagent for studying metabolism, energy regulation, and glycolytic pathways. The encoded protein, glyceraldehyde 3 phosphate dehydrogenase (GAPDH), is a central enzyme in glycolysis that catalyzes the sixth step of glucose breakdown. Its role in producing ATP and NADH links it directly to cellular energy homeostasis.
GAPDH is expressed at high levels in most tissues, reflecting the universal demand for glycolysis. In rapidly dividing cells, GAPDH expression is elevated to support anabolic growth and biosynthetic demands. Cancer cells, in particular, upregulate GAPDH as part of the Warburg effect, highlighting its significance in tumor metabolism. Detection with GAPDH antibody enables researchers to monitor glycolytic activity and metabolic adaptation in diverse disease contexts.
Beyond its role in glycolysis, GAPDH influences metabolic signaling pathways. It interacts with proteins involved in glucose sensing, mitochondrial function, and apoptosis. GAPDH can translocate to the mitochondria or nucleus during stress, linking metabolic flux to cell survival decisions. This dual role in energy production and signaling makes GAPDH a hub in cell biology.
Structurally, GAPDH consists of four identical subunits, each with catalytic and NAD binding domains. Its conserved architecture explains why GAPDH antibody can detect the protein in multiple organisms, including human, mouse, and yeast. This conservation ensures that studies of metabolic regulation can be translated across models.
The GAPDH antibody is commonly used in western blotting, immunofluorescence, immunohistochemistry, and ELISA to detect protein abundance and localization. These applications are critical for cancer biology, metabolic disease research, and fundamental energy metabolism studies. For scientists investigating glycolysis, metabolic adaptation, or bioenergetics, the GAPDH antibody provides a specific and dependable detection tool. NSJ Bioreagents offers validated antibodies designed to ensure reproducibility and accuracy in advanced metabolic research.
Optimal dilution of the GAPDH antibody should be determined by the researcher.
Amino acids N136-E335 from the human protein were used as the immunogen for the GAPDH antibody.
After reconstitution, the GAPDH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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