- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GAPDH Antibody / HRP Conjugate [clone A-7] (RQ4477)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ4477 | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA | 100 ul | 439 |
|
| Availability | 1-2 weeks |
| Species Reactivity | Human, Mouse, Rat |
| Format | HRP Conjugate |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | A-7 |
| Purity | Protein A affinity |
| UniProt | P04406 |
| Localization | Cytoplasm, Nucleus, Membrane |
| Applications | Western Blot : 1:5000-1:20000 |
| Limitations | This GAPDH antibody is available for research use only. |
|
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GAPDH antibody is a critical reagent for investigating cancer metabolism, glycolysis, and tumor biology. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes an essential glycolytic reaction, generating energy and biosynthetic precursors for cell growth. Its elevated expression in tumors reflects the metabolic reprogramming known as the Warburg effect, where cancer cells rely heavily on glycolysis even in oxygen rich conditions.
In cancer research, GAPDH serves both as a marker of metabolic state and as a reference protein in experimental assays. High expression of GAPDH supports biosynthetic demands of rapidly dividing cells and correlates with aggressiveness in certain cancers. The GAPDH antibody is therefore used to monitor expression changes under therapeutic interventions or metabolic stress.
Beyond cancer, GAPDH participates in apoptosis and autophagy, making it a multifunctional protein in cell fate regulation. Nuclear accumulation of GAPDH has been linked to pro apoptotic signaling during oxidative stress. Conversely, cytoplasmic GAPDH supports survival pathways, highlighting its dual role in promoting or inhibiting tumor progression depending on context.
Molecularly, GAPDH is a tetramer with NAD binding sites and conserved catalytic cysteine residues. Its enzymatic mechanism involves oxidation and phosphorylation reactions that are critical for maintaining glycolytic flux. This structural stability enables GAPDH antibody detection across different organisms and experimental systems.
The GAPDH antibody is widely used in western blotting, immunohistochemistry, ELISA, and immunofluorescence. These applications allow detection in tumor tissues, cell lines, and experimental models, providing insights into cancer metabolism and therapy resistance. For oncologists and molecular biologists, the GAPDH antibody is a specific and reliable tool. NSJ Bioreagents supplies validated antibodies that guarantee reproducibility and accuracy in advanced cancer research.
Optimal dilution of the GAPDH antibody should be determined by the researcher.
A synthetic peptide from human GAPDH was used as the immunogen for the GAPDH antibody.
Store the GAPDH antibody at -20oC.
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