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Home >> Antibodies >> GAPDH Antibody

GAPDH Antibody [clone 1A10A10] (F53489)

  Catalog No Formulation Size Price (USD)  
Image F53489-0.1ML In ascites with 0.09% sodium azide 0.1 ml 329
Western blot analysis of GAPDH antibody and HepG2, A375, A2058, NCI-H460 and CEM lysate (1:25000 dilution). Predicted molecular weight ~36kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with GAPDH antibody
Confocal immunofluorescent analysis of GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 1A10A10
Purity Ascites
UniProt P04406
Applications Western blot : 1:5000-25000
IHC (Paraffin) : 1:1000-1:10000
Immunofluorescence : 1:10-1:50
Limitations This GAPDH antibody is available for research use only.
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Description

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

Application Notes

Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Recombinant protein was used to produce this GAPDH antibody.

Storage

Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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