- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GAMT Antibody / Guanidinoacetate N-methyltransferase (FY12646)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12646 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 449 |
|
| Availability | 1-2 days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Lyophilized |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Immunogen affinity purified |
| Buffer | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| UniProt | Q14353 |
| Applications | Western Blot : 0.25-0.5ug/ml ELISA : 0.1-0.5ug/ml |
| Limitations | This GAMT antibody is available for research use only. |
|
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GAMT antibody detects Guanidinoacetate N-methyltransferase, an enzyme that catalyzes the final step in the biosynthesis of creatine, a key molecule for cellular energy storage and transfer. GAMT converts guanidinoacetate and S-adenosyl-L-methionine into creatine and S-adenosyl-L-homocysteine, a critical reaction for maintaining high-energy phosphate metabolism in muscle and neural tissues. The GAMT antibody is widely used in metabolism, neurology, and biochemical research to study creatine biosynthesis, methylation pathways, and energy regulation.
GAMT is encoded by the GAMT gene located on human chromosome 19p13.3. The protein is approximately 236 amino acids long and localizes to the cytoplasm and mitochondria of metabolically active cells such as skeletal muscle fibers, hepatocytes, and neurons. GAMT belongs to the family of methyltransferases and functions as a homodimer, with each subunit contributing to catalytic activity and substrate specificity.
The GAMT antibody detects a 28 kilodalton protein by western blot and shows cytosolic and mitochondrial localization by immunofluorescence microscopy. Creatine synthesized by GAMT is essential for buffering cellular ATP levels through the phosphocreatine shuttle system, particularly in tissues with fluctuating energy demands. In the brain, creatine supports neuronal excitability and synaptic transmission, while in muscle, it sustains rapid contraction and recovery.
Deficiency of GAMT causes guanidinoacetate methyltransferase deficiency, a rare inborn error of metabolism characterized by creatine depletion in the brain, developmental delay, seizures, and movement disorders. Accumulation of guanidinoacetate is neurotoxic, leading to oxidative stress and neurotransmission imbalance. Early diagnosis through enzyme assays or molecular testing is critical, as dietary creatine supplementation can mitigate neurological symptoms.
Beyond its metabolic role, GAMT activity influences methylation capacity, homocysteine metabolism, and nitric oxide synthesis, linking it to broader aspects of cellular function. Altered GAMT expression has been associated with cardiovascular disease, neurodegeneration, and aging due to impaired energy homeostasis and methyl donor imbalance.
Because GAMT is central to energy metabolism and methyl group regulation, it serves as an important biomarker for studying metabolic diseases and neurological function. NSJ Bioreagents provides a validated GAMT antibody optimized for its applications, supporting research into creatine metabolism, mitochondrial function, and energy regulation.
Optimal dilution of the GAMT antibody should be determined by the researcher.
E.coli-derived human GAMT recombinant protein (Position: M1-G236) was used as the immunogen for the GAMT antibody.
After reconstitution, the GAMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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