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Home >> Antibodies >> Gamma Enolase Antibody / NSE / ENO2

Gamma Enolase Antibody / NSE / ENO2 [clone ENO2/9047] (V5453)

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  Catalog No Formulation Size Price (USD)  
Image V5453-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5453-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5453SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human pancreas tissue with Gamma Enolase antibody (clone ENO2/9047). Inset: PBS used in place of primary Ab (secondary Ab negative control).
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IHC staining of FFPE human pancreas tissue with Gamma Enolase antibody (clone ENO2/9047). Inset: PBS used in place of primary Ab (secondary Ab negative control).
SDS-PAGE analysis of purified, BSA-free Gamma Enolase antibody (clone ENO2/9047) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name ENO2/9047
Purity Protein A/G affinity
UniProt P09104
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Gamma Enolase antibody is available for research use only.
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Description

This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found predominantly in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin, ?Chromogranin A, and Neurofilament.

Application Notes

Optimal dilution of the Gamma Enolase antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human NSE gamma (within amino acids 150-300) was used as the immunogen for the Gamma Enolase antibody.

Storage

Aliquot the Gamma Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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