GALNT7 Antibody / O-Glycosylation Enzyme Antibody (FY12126)

GALNT7 Antibody Human Lung Cancer IF. Immunofluorescence analysis of FFPE human lung cancer tissue using GALNT7 Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (5 ug/mL) at 4°C. Cy3-conjugated goat anti-rabbit IgG (red) was used for detection, and DAPI (blue) was used as a nuclear counterstain. Strong cytoplasmic staining with prominent perinuclear enrichment is observed in malignant epithelial cells, consistent with the Golgi localization of GALNT7, an enzyme that initiates mucin-type O-linked glycosylation. These results support the use of GALNT7 Antibody / O-Glycosylation Enzyme Antibody for studying GALNT7 expression, Golgi organization and protein O-glycosylation.

GALNT7 Antibody Human Lung Cancer IHC. Immunohistochemistry analysis of FFPE human lung cancer tissue using GALNT7 Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly cytoplasmic staining with focal perinuclear enhancement is observed in malignant epithelial cells, consistent with the Golgi localization of GALNT7, an enzyme that initiates mucin-type O-linked glycosylation. These results support the use of GALNT7 Antibody / O-Glycosylation Enzyme Antibody for studying GALNT7 expression, protein O-glycosylation and cancer-associated glycosylation pathways.

GALNT7 Antibody Human Prostate Cancer IF. Immunofluorescence analysis of FFPE human prostate cancer tissue using GALNT7 Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (5 ug/mL) at 4°C. Cy3-conjugated goat anti-rabbit IgG (red) was used for detection, and DAPI (blue) was used as a nuclear counterstain. Strong cytoplasmic staining with prominent perinuclear enrichment is observed in malignant glandular epithelial cells, consistent with the Golgi localization of GALNT7, an enzyme that initiates mucin-type O-linked glycosylation. These results support the use of GALNT7 Antibody / O-Glycosylation Enzyme Antibody for studying GALNT7 expression, Golgi organization and protein O-glycosylation.

GALNT7 Antibody Human Prostate Cancer IHC. Immunohistochemistry analysis of FFPE human prostate cancer tissue using GALNT7 Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly cytoplasmic staining with perinuclear enhancement is observed in malignant glandular epithelial cells, consistent with the Golgi localization of GALNT7, an enzyme that initiates mucin-type O-linked glycosylation. These results support the use of GALNT7 Antibody / O-Glycosylation Enzyme Antibody for studying GALNT7 expression, protein O-glycosylation and cancer-associated glycosylation pathways.

GALNT7 Antibody HeLa Cell IF. Immunofluorescence analysis of HeLa cells using GALNT7 Antibody (green) and alpha tubulin antibody (red). Cells were treated with enzyme antigen retrieval reagent for 15 minutes prior to blocking with goat serum and overnight incubation with GALNT7 Antibody (5 ug/mL) and alpha tubulin antibody at 4°C. Fluoro488- and Cy3-conjugated secondary antibodies were used for detection, and DAPI (blue) was used as a nuclear counterstain. GALNT7 displays a concentrated perinuclear staining pattern consistent with Golgi localization, while alpha tubulin highlights the cytoskeletal network. These results support the use of GALNT7 Antibody / O-Glycosylation Enzyme Antibody for studying GALNT7 expression, Golgi organization and protein O-glycosylation.

Western blot analysis of GALNT7 using anti-GALNT7 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT7 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for GALNT7 at approximately 75 kDa. The expected band size for GALNT7 is at 75 kDa.

GALNT7 Antibody HaCaT Cell IP. Immunoprecipitation of GALNT7 from HaCaT whole cell lysate using GALNT7 Antibody, followed by Western blot analysis with the same antibody. Lane 1: HaCaT input lysate (30 ug); Lane 2: rabbit control IgG immunoprecipitation; Lane 3: GALNT7 immunoprecipitation using 2 ug of GALNT7 Antibody from 500 ug of HaCaT lysate. A specific band is detected at approximately 75 kDa in the input and GALNT7 immunoprecipitation samples, while the control IgG lane lacks a corresponding GALNT7 band. The band at approximately 50 kDa represents the IgG heavy chain derived from the immunoprecipitation procedure. These results support the use of GALNT7 Antibody / O-Glycosylation Enzyme Antibody for immunoprecipitation, protein interaction studies and investigations of O-glycosylation pathways.