Galectin 1 Antibody (R32841)

Galectin 1 Antibody Mouse and Rat Tissue WB. Western blot analysis of 1) mouse thymus, 2) mouse kidney, 3) mouse testis, 4) mouse ovary, 5) rat heart, 6) rat skeletal muscle, 7) rat stomach, and 8) rat testis tissue lysates using Galectin 1 antibody at 0.5 ug/ml. A prominent immunoreactive band is detected at approximately 15 kDa in all samples, consistent with the predicted molecular weight of Galectin-1 (LGALS1). Galectin-1 is a beta-galactoside-binding lectin involved in immune regulation, cell adhesion, apoptosis, extracellular matrix remodeling, and glycan-mediated signaling pathways. The consistent detection of endogenous Galectin-1 across multiple mouse and rat tissues demonstrates the broad species reactivity and utility of this antibody for western blot applications. Predicted molecular weight: ~15 kDa. An additional higher molecular weight band is observed in some tissues, which may reflect tissue-specific isoforms, oligomeric forms, or post-translationally modified Galectin-1 species.

Galectin 1 Antibody Rat Kidney IHC. Immunohistochemical staining of FFPE rat kidney tissue using Galectin 1 antibody at 1 ug/ml demonstrates cytoplasmic and membranous HRP-DAB brown staining within renal tubular epithelial cells and associated kidney structures. The observed staining pattern is consistent with expression of Galectin-1 (LGALS1), a beta-galactoside-binding lectin involved in cell adhesion, immune regulation, extracellular matrix remodeling, tissue repair, and glycan-mediated signaling pathways. Positive immunoreactivity is primarily localized to tubular compartments, supporting the known role of Galectin-1 in renal physiology and cellular communication within the kidney microenvironment. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Galectin 1 Antibody Rat Small Intestine IHC. Immunohistochemical staining of FFPE rat small intestine tissue using Galectin 1 antibody at 1 ug/ml demonstrates cytoplasmic and membranous HRP-DAB brown staining within intestinal epithelial cells and mucosal-associated cellular structures. The observed staining pattern is consistent with expression of Galectin-1 (LGALS1), a beta-galactoside-binding lectin involved in cell adhesion, immune regulation, epithelial homeostasis, extracellular matrix interactions, and glycan-mediated signaling pathways. Positive immunoreactivity is present within the intestinal mucosa, supporting the role of Galectin-1 in maintaining gastrointestinal tissue integrity and cellular communication within the intestinal microenvironment. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Galectin 1 Antibody Mouse Kidney IHC. Immunohistochemical staining of FFPE mouse kidney tissue using Galectin 1 antibody at 1 ug/ml demonstrates cytoplasmic and membranous HRP-DAB brown staining throughout renal tubular epithelial cells, with additional staining present in selected glomerular and perivascular-associated structures. The observed staining pattern is consistent with expression of Galectin-1 (LGALS1), a beta-galactoside-binding lectin involved in cell adhesion, immune regulation, extracellular matrix remodeling, tissue repair, and glycan-mediated signaling pathways. Positive immunoreactivity within multiple renal compartments supports the role of Galectin-1 in kidney homeostasis and cellular communication within the renal microenvironment. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.