Galectin 1 Antibody (R32841)

Galectin 1 Antibody Human Colon Cancer IHC. Immunohistochemistry was performed on FFPE human colon cancer tissue using Galectin 1 Antibody. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was visualized using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed in the malignant epithelial cells and surrounding stromal components, consistent with the known extracellular matrix and tumor-associated expression pattern of Galectin 1. These results demonstrate that this Galectin 1 Antibody is suitable for immunohistochemical detection of Galectin 1 in formalin-fixed, paraffin-embedded human colon cancer tissue.

Galectin 1 Antibody A549 Cell IF. Immunofluorescence was performed on A549 cells using Galectin 1 Antibody. Enzyme antigen retrieval was carried out with AR0022 for 15 minutes prior to incubation with the primary antibody at 5 ug/ml overnight at 4°C. A Fluoro594-conjugated goat anti-rabbit IgG secondary antibody (red) was used for detection, and nuclei were counterstained with DAPI (blue). Predominantly cytoplasmic staining is observed throughout the A549 cells, consistent with the expected intracellular localization of Galectin 1. These results demonstrate that this Galectin 1 Antibody is suitable for immunofluorescent detection of Galectin 1 in cultured human cells.

Galectin 1 Antibody Mouse Kidney IHC. Immunohistochemistry was performed on FFPE mouse kidney tissue using Galectin 1 Antibody. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was visualized using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Predominantly cytoplasmic staining is observed in renal tubular epithelial cells, with additional staining of selected tubular structures, consistent with the reported expression pattern of Galectin 1 in kidney tissue. These results demonstrate that this Galectin 1 Antibody is suitable for immunohistochemical detection of Galectin 1 in formalin-fixed, paraffin-embedded mouse kidney tissue.

Galectin 1 Antibody Mouse Intestine IHC. Immunohistochemistry was performed on FFPE mouse intestine tissue using Galectin 1 Antibody. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was visualized using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Predominantly cytoplasmic staining is observed within the intestinal mucosa and surrounding stromal cells, consistent with the known expression pattern of Galectin 1 in gastrointestinal tissues. These results demonstrate that this Galectin 1 Antibody is suitable for immunohistochemical detection of Galectin 1 in formalin-fixed, paraffin-embedded mouse intestine tissue.

Galectin 1 Antibody Rat Kidney IHC. Immunohistochemistry was performed on FFPE rat kidney tissue using Galectin 1 Antibody. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was visualized using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Predominantly cytoplasmic staining is observed in renal glomeruli and selected tubular epithelial cells, consistent with the reported distribution of Galectin 1 in kidney tissue. These results demonstrate that this Galectin 1 Antibody is suitable for immunohistochemical detection of Galectin 1 in formalin-fixed, paraffin-embedded rat kidney tissue.

Galectin 1 Antibody Human, Rat and Mouse WB. Western blot analysis was performed using Galectin 1 Antibody. Lane 1: human HeLa whole cell lysate; lane 2: human A549 whole cell lysate; lane 3: human A375 whole cell lysate; lane 4: human MCF-7 whole cell lysate; lane 5: rat kidney tissue lysate; lane 6: rat PC-12 whole cell lysate; lane 7: mouse kidney tissue lysate; lane 8: mouse NIH/3T3 whole cell lysate. Proteins were separated on a 13% SDS-PAGE gel, transferred to a nitrocellulose membrane, and incubated with the primary antibody at 0.5 ug/ml overnight at 4°C. A specific immunoreactive band is detected at approximately 15 kDa in all tested samples, consistent with the expected molecular weight of Galectin 1. These results demonstrate that this Galectin 1 Antibody is suitable for Western blot detection of Galectin 1 across human, rat, and mouse tissues and cell lines.