Galectin 1 Antibody / Immune Regulation Protein Antibody [clone GAL1/1831] (V3201)

Galectin 1 Antibody Human JEG-3 and K562 WB. Western blot analysis of 1) human JEG-3 and 2) human K562 cell lysates using Galectin 1 antibody (clone GAL1/1831). A strong, distinct band is detected at approximately 14 kDa in both cell lines, consistent with the expected molecular weight of Galectin-1 (LGALS1), a beta-galactoside-binding lectin involved in immune regulation, cell adhesion, angiogenesis, and tumor microenvironment signaling. The results demonstrate specific detection of endogenous Galectin-1 protein and support the utility of clone GAL1/1831 for western blot applications. Predicted molecular weight: ~14 kDa.

Galectin 1 Antibody HeLa WB. Western blot analysis of human HeLa cell lysate using Galectin 1 antibody (clone GAL1/1831). A distinct immunoreactive band is observed at approximately 14 kDa, corresponding to the expected molecular weight of Galectin-1 (LGALS1). Galectin-1 is a beta-galactoside-binding lectin that participates in immune regulation, cell-cell interactions, apoptosis, angiogenesis, and tumor microenvironment signaling. The clean banding pattern supports the specificity of clone GAL1/1831 for detection of endogenous Galectin-1 by western blot. Predicted molecular weight: ~14 kDa.

Galectin 1 Antibody Human Cell Line WB. Western blot analysis of 1) human HeLa, 2) human K562, and 3) human HEK293 cell lysates using Galectin 1 antibody (clone GAL1/1831). A strong, discrete band is detected at approximately 14 kDa in all three cell lines, consistent with the expected molecular weight of Galectin-1 (LGALS1). Galectin-1 is a beta-galactoside-binding lectin involved in immune regulation, cell adhesion, apoptosis, angiogenesis, and tumor microenvironment remodeling. The consistent detection of a single prominent band across multiple human cell lines supports the specificity and reliability of clone GAL1/1831 for western blot-based analysis of endogenous Galectin-1 expression. Predicted molecular weight: ~14 kDa.

Galectin 1 Antibody HeLa IF. Immunofluorescent staining of permeabilized human HeLa cells using Galectin 1 antibody (clone GAL1/1831, green) demonstrates prominent cytoplasmic staining with a finely granular distribution throughout the cell body. The observed pattern is consistent with the known intracellular localization of Galectin-1 (LGALS1), a beta-galactoside-binding lectin involved in immune regulation, cell adhesion, apoptosis, and extracellular matrix interactions. RedDot nuclear counterstain (red) clearly delineates cell nuclei, while Galectin-1 immunoreactivity is primarily excluded from the nuclear compartment and enriched within the cytoplasm. These results support the utility of clone GAL1/1831 for immunofluorescent detection of endogenous Galectin-1 expression in cultured human cells.

Galectin 1 Antibody Prostate Carcinoma IHC. Immunohistochemical staining of FFPE human prostate carcinoma tissue using Galectin 1 antibody (clone GAL1/1831) demonstrates strong cytoplasmic and stromal-associated HRP-DAB brown staining within the tumor microenvironment. Prominent immunoreactivity is observed in spindle-shaped stromal cells surrounding malignant glandular structures, consistent with the known expression pattern of Galectin-1 (LGALS1), a beta-galactoside-binding lectin involved in immune regulation, extracellular matrix remodeling, angiogenesis, and tumor progression. The staining pattern highlights both stromal and tumor-associated compartments and supports the use of clone GAL1/1831 for detection of Galectin-1 expression in FFPE tissue specimens. HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Galectin 1 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Galectin 1 antibody (clone GAL1/1831) demonstrates exceptional target specificity for Galectin-1 (LGALS1). The GAL1/1831 monoclonal antibody identifies Galectin-1 as the highest-ranking target with a Z-score of 127.95 and an S-score of 103.73, greatly exceeding all non-target proteins present on the array. These results confirm the foremost specificity of clone GAL1/1831 and provide strong evidence for selective recognition of Galectin-1 with minimal off-target reactivity. Z-score represents the signal strength generated by antibody binding to a specific protein relative to all proteins present on the array, while S-score reflects the specificity gap between the intended target and the next highest-ranking protein. An S-score greater than 2.5 is generally considered indicative of highly specific target recognition. The exceptionally high Z- and S-scores obtained for GAL1/1831 support its suitability for applications requiring accurate and reliable detection of Galectin-1 expression.

SDS-PAGE analysis of purified, BSA-free Galectin 1 antibody (clone GAL1/1831) as confirmation of integrity and purity.