GALC Antibody / Galactocerebrosidase Antibody (FY13064)

GALC Antibody Human Neuroblastoma and Rodent Brain WB. Western blot analysis of GALC expression in multiple human cell and rodent tissue lysates. Lane 1: human U-373 whole cell lysate. Lane 2: human U-251 whole cell lysate. Lane 3: human SH-SY5Y whole cell lysate. Lane 4: human HEL whole cell lysate. Lane 5: rat brain tissue lysate. Lane 6: mouse brain tissue lysate. A single major band is detected at approximately 100 kDa in all samples using GALC antibody, consistent with the well-characterized N-glycosylated precursor form of Galactocerebrosidase. Although the predicted molecular weight of GALC is approximately 77 kDa, the mature enzyme is synthesized as a heavily glycosylated precursor of approximately 100 kDa that is subsequently processed into smaller lysosomal subunits. This result demonstrates that the GALC Antibody / Galactocerebrosidase Antibody is suitable for Western blot detection of endogenous GALC in human, rat, and mouse samples.

GALC Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue using GALC antibody at 2 ug/ml following heat-induced epitope retrieval in pH8 EDTA buffer. Detection was performed using an HRP-conjugated secondary antibody with DAB chromogen. GALC immunoreactivity is observed throughout the brain parenchyma with predominantly cytoplasmic staining in neural cells, consistent with the lysosomal localization of Galactocerebrosidase, an essential enzyme involved in galactolipid metabolism and myelin maintenance. This result demonstrates that the GALC Antibody / Galactocerebrosidase Antibody is suitable for immunohistochemical detection of endogenous GALC in formalin-fixed, paraffin-embedded mouse tissue.