GAD67 Antibody (R30213)

GAD67 Antibody Human Cerebellum IHC. Immunohistochemistry analysis of FFPE human cerebellum tissue using GAD67 Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Strong cytoplasmic staining is observed throughout the cerebellar cortex, highlighting GABAergic neurons consistent with the expression of GAD67 (glutamate decarboxylase 67), the enzyme responsible for gamma-aminobutyric acid (GABA) synthesis. These results support the use of GAD67 Antibody for studying GAD67 expression, GABAergic neurotransmission and inhibitory neuronal biology.

GAD67 Antibody Rodent Brain WB. Western blot analysis using GAD67 Antibody. Lane 1: rat brain tissue lysate; Lane 2: mouse brain tissue lysate. A specific band is detected at approximately 67 kDa in both samples, consistent with the expected molecular weight of GAD67 (GAD1). The strong expression observed in rodent brain tissue is consistent with the abundant presence of GAD67 in GABAergic neurons, where it catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). These results support the use of GAD67 Antibody for studying GAD67 expression, GABA synthesis and inhibitory neurotransmission.

GAD67 Antibody SiHa Cell IF. Immunofluorescence analysis of SiHa cells using GAD67 Antibody. Cells were treated with enzyme antigen retrieval reagent for 15 minutes prior to blocking with goat serum and overnight incubation with primary antibody (5 ug/mL) at 4°C. Fluoro488-conjugated secondary antibody was used for detection, and DAPI (blue) was used as a nuclear counterstain. Diffuse cytoplasmic staining is observed throughout the cells, consistent with the intracellular localization of GAD67 (glutamate decarboxylase 67), the enzyme responsible for gamma-aminobutyric acid (GABA) synthesis. These results support the use of GAD67 Antibody for studying GAD67 expression, GABA biosynthesis and neuronal marker biology.