GAD2 Antibody / Glutamate Decarboxylase 2 Antibody [clone 7G2] (RQ5856)

GAD2 Antibody HeLa Immunofluorescence. Immunofluorescent analysis of formalin-fixed human HeLa cells stained with GAD2 Antibody clone 7G2 demonstrates diffuse cytoplasmic green fluorescence consistent with Glutamate decarboxylase 2 / GAD65-associated inhibitory neurotransmitter synthesis pathways. This Glutamate decarboxylase 2 antibody highlights GABA synthesis-associated signaling and neuronal enzyme expression patterns within epithelial-derived cells. Nuclei are counterstained with DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min prior to antibody incubation.

GAD2 Antibody Glioma IHC. Immunohistochemistry analysis of FFPE human glioma tissue stained with mouse monoclonal GAD2 Antibody clone 7G2 demonstrates focal cytoplasmic HRP-DAB brown staining within scattered neural-associated tumor cell populations, consistent with Glutamate decarboxylase 2 / GAD65-associated inhibitory neurotransmitter synthesis pathway expression. This Glutamate decarboxylase 2 antibody highlights GABAergic signaling-associated cellular regulation and neural differentiation biology within glioma tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

GAD2 Antibody Brain WB. Western blot analysis of 1) rat brain and 2) mouse brain lysates using mouse monoclonal GAD2 Antibody clone 7G2 detects strong bands at approximately 65 kDa, consistent with the predicted molecular weight of Glutamate decarboxylase 2 / GAD65. This Glutamate decarboxylase 2 antibody highlights abundant expression of inhibitory neurotransmitter-associated signaling machinery within central nervous system tissue and supports characterization of GABAergic neuronal differentiation pathways.

GAD2 Antibody 293T FACS. Flow cytometry analysis of human 293T cells stained with GAD2 Antibody clone 7G2 demonstrates a distinct right-shifted fluorescence population relative to the isotype control, consistent with Glutamate decarboxylase 2 / GAD65-associated inhibitory neurotransmitter synthesis pathway expression. This Glutamate decarboxylase 2 antibody supports characterization of GABA synthesis-associated signaling and neuronal differentiation-related protein expression in human cells. Red=cells alone, Green=isotype control, Blue=GAD2 antibody.

GAD2 Antibody U-2 OS FACS. Flow cytometry analysis of human U-2 OS cells stained with GAD2 Antibody clone 7G2 demonstrates a distinct right-shifted fluorescence population relative to the isotype control, consistent with Glutamate decarboxylase 2 / GAD65-associated inhibitory neurotransmitter synthesis pathway expression. This Glutamate decarboxylase 2 antibody supports characterization of GABA synthesis-associated signaling and neuronal differentiation-related protein expression in osteosarcoma-derived cells. Red=cells alone, Green=isotype control, Blue=GAD2 antibody.