GAD1 Antibody / Glutamate decarboxylase 1 / GAD67 [clone BICCN-GAD67-1A6] (V5904)

Formalin-fixed, paraffin-embedded human brain stained with GAD1/Glutamate decarboxylase 1 antibody (clone BICCN-GAD67-1A6). Diffuse cytoplasmic brown chromogenic staining is observed in neuronal cell bodies and processes, consistent with GABAergic neuron labeling, while surrounding nuclei are counterstained blue. Inset shows a PBS-only negative control processed without primary antibody, demonstrating minimal non-specific background staining.

Western blot analysis of human brain tissue lysate using GAD1/Glutamate decarboxylase 1 antibody (clone BICCN-GAD67-1A6). Expected molecular weight ~67 kDa.

Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD1/Glutamate decarboxylase 1 antibody (clone BICCN-GAD67-1A6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE analysis of purified GAD1/Glutamate decarboxylase 1 antibody (clone BICCN-GAD67-1A6). Confirmation of Purity and Integrity of Antibody.