FZD9 Antibody / Frizzled-9 Antibody (FY13537)

FZD9 Antibody by IF in human testis. Immunofluorescence analysis of paraffin-embedded human testis using Frizzled-9 Antibody reveals red fluorescence in cells lining seminiferous tubules, with blue DAPI-stained nuclei. The staining is evident across multiple tubule profiles and is associated with the seminiferous epithelium. Heat-mediated antigen retrieval used EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 5 ug/mL overnight at 4oC and detected with a Cy3-conjugated secondary antibody at 1:500. This figure illustrates FZD9-associated immunoreactivity in human testicular tissue.

FZD9 Antibody by IF in human breast carcinoma. Immunofluorescence staining of paraffin-embedded human breast carcinoma with Frizzled-9 Antibody reveals red fluorescence within epithelial tumor structures and blue DAPI nuclear counterstaining. The signal outlines numerous cells within the gland-forming tumor compartment. Tissue underwent heat-mediated epitope retrieval in EDTA buffer at pH 8.0 and blocking with 10% goat serum. Primary antibody was applied at 5 ug/mL overnight at 4oC, followed by a Cy3-conjugated secondary antibody at 1:500. The image demonstrates FZD9-associated staining in the carcinoma section.

FZD9 Antibody by WB in rat and mouse tissues. Western blot analysis with Frizzled-9 Antibody detects a principal band near 70 kDa in rat small intestine (lane 1), rat testis (lane 2), mouse small intestine (lane 3), and mouse testis (lane 4). The observed migration is moderately above the calculated approximately 64.5 kDa molecular weight of FZD9. Reduced tissue lysates containing 30 ug protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. Primary antibody was incubated at 0.5 ug/mL overnight at 4oC, followed by HRP-based chemiluminescent detection. The principal immunoreactive band is present in both species.

FZD9 Antibody by IHC in human testis. Immunohistochemistry of paraffin-embedded human testis using Frizzled-9 Antibody demonstrates brown immunoreactivity among cells of the seminiferous tubules, with blue hematoxylin-stained nuclei. Positive staining is distributed along portions of the seminiferous epithelium. Sections underwent EDTA antigen retrieval at pH 8.0, blocking with 10% goat serum, and incubation with primary antibody at 2 ug/mL overnight at 4oC. Bound antibody was visualized using a peroxidase-linked secondary reagent and DAB chromogen. The image provides histological evidence of FZD9-associated staining in testicular tissue.

FZD9 Antibody by IHC in human breast carcinoma. Immunohistochemistry of paraffin-embedded human breast carcinoma using Frizzled-9 Antibody shows brown immunoreactivity in a subset of epithelial tumor cells, with hematoxylin counterstaining highlighting nuclei. The staining varies across the glandular structures visible in the section. Heat-mediated antigen retrieval was performed in EDTA buffer at pH 8.0, followed by 10% goat serum blocking. Primary antibody was applied at 2 ug/mL overnight at 4oC and detected with a peroxidase-conjugated secondary antibody and DAB. This figure documents FZD9-associated immunoreactivity in human breast carcinoma.

FZD9 Antibody by FACS in THP-1 cells. Flow cytometric analysis using Frizzled-9 Antibody shows a rightward fluorescence shift in stained THP-1 cells (blue histogram) compared with the rabbit IgG isotype control (green) and unstained control (red). Cells were fixed in 4% paraformaldehyde, blocked with 10% goat serum, and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. DyLight 488-conjugated secondary antibody was used for detection. The histogram demonstrates antibody-associated fluorescence under the stated conditions.