FZD1 Antibody / Frizzled 1 Antibody (R32354)

FZD1 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using FZD1 Antibody at 2 ug/ml with HRP/DAB detection following heat-induced epitope retrieval in pH8 EDTA buffer. Moderate membranous and cytoplasmic staining is observed in the tumor cells, consistent with the expected localization of the Frizzled 1 receptor. This result demonstrates the utility of FZD1 Antibody for investigating Frizzled 1 expression and Wnt signaling receptor distribution in human cancer tissues.

FZD1 Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placenta tissue using FZD1 Antibody at 2 ug/ml with HRP/DAB detection following heat-induced epitope retrieval in pH8 EDTA buffer. Distinct membranous and cytoplasmic staining is observed in placental trophoblast cells, consistent with the expected localization of the Frizzled 1 receptor. This staining pattern demonstrates the utility of FZD1 Antibody for investigating Frizzled 1 expression and Wnt signaling receptor distribution in human tissues.

FZD1 Antibody Human, Mouse and Rat WB. Western blot analysis of FZD1 Antibody using lysates from lane 1: human HeLa cells, lane 2: human Jurkat cells, lane 3: human Ramos cells, lane 4: human RT4 cells, lane 5: rat liver, lane 6: rat heart, lane 7: mouse liver and lane 8: mouse heart. A specific band is detected at approximately 71 kDa, consistent with the predicted molecular weight of FZD1. These results demonstrate cross-species detection of endogenous Frizzled 1 and support the use of FZD1 Antibody for investigating Wnt receptor expression and signaling in human and rodent tissues.

FZD1 Antibody Human MCF-7 FACS. Flow cytometry analysis of fixed MCF-7 human breast cancer cells stained with FZD1 Antibody at 1 ug/10^6 cells, followed by a Fluoro488-conjugated secondary antibody. The blue histogram (FZD1) shows a clear rightward shift compared with the rabbit IgG isotype control (green) and unstained cells (red), demonstrating specific detection of endogenous Frizzled 1 expression. These results support the use of FZD1 Antibody for analyzing FZD1 expression and Wnt signaling receptor distribution by flow cytometry.