FXN Antibody / Frataxin (FY13347)

FXN Antibody Ovarian Carcinoma IHC. Immunohistochemical staining of FFPE human ovarian carcinoma tissue using anti-FXN antibody demonstrates strong granular cytoplasmic HRP-DAB brown staining within malignant gland-forming epithelial cells, consistent with mitochondrial localization of Frataxin / FXN in metabolically active tumor-associated cellular populations. The observed staining pattern supports the established role of FXN in mitochondrial iron homeostasis and oxidative metabolism-associated pathways. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum followed by incubation with 2 ug/ml rabbit anti-FXN antibody overnight at 4°C. Peroxidase-conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 min at 37°C before HRP-DAB visualization.

FXN Antibody Multi-Cell Line WB. Western blot analysis of human HepG2, HEL, 293T, and K562 whole cell lysates using anti-FXN antibody demonstrates consistent bands near 13-15 kDa across all tested cell lines, corresponding to the mature processed form of Frataxin / FXN following mitochondrial import-associated cleavage. The observed expression profile supports widespread cellular distribution of this mitochondrial iron metabolism protein and aligns with the established role of FXN in iron-sulfur cluster biogenesis and oxidative phosphorylation-associated energy regulation. Electrophoresis was performed on a 12% SDS-PAGE gel followed by transfer to nitrocellulose membrane and HRP-ECL detection.

FXN Antibody Pancreatic Adenocarcinoma IHC. Immunohistochemical staining of FFPE human pancreatic carcinoma tissue using anti-FXN antibody demonstrates extensive granular cytoplasmic HRP-DAB brown staining within malignant glandular epithelial cells, consistent with mitochondrial localization of Frataxin / FXN in metabolically active tumor-associated cellular compartments. The observed staining pattern supports the established role of FXN in mitochondrial iron homeostasis, oxidative phosphorylation, and iron-sulfur cluster-associated metabolic pathways. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum followed by overnight incubation with 2 ug/ml rabbit anti-FXN antibody at 4°C. Peroxidase-conjugated goat anti-rabbit IgG was used as secondary antibody prior to HRP-DAB chromogenic detection.

FXN Antibody Mitochondrial Tumor Marker IHC. Immunohistochemical staining of FFPE human pancreatic cancer tissue using anti-FXN antibody reveals strong granular cytoplasmic HRP-DAB brown staining within malignant epithelial glands and clustered tumor-associated cellular structures. The punctate staining distribution is consistent with mitochondrial localization of Frataxin / FXN and supports its established involvement in oxidative metabolism and mitochondrial iron regulation within high energy-demand carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0). Tissue sections were blocked with 10% goat serum prior to overnight incubation with 2 ug/ml rabbit anti-FXN antibody at 4°C, followed by HRP-conjugated secondary antibody and DAB chromogenic visualization.

FXN Antibody HepG2 FACS. Flow cytometry analysis of fixed and permeabilized human HepG2 cells using anti-FXN antibody demonstrates a pronounced rightward fluorescence shift relative to isotype control, supporting intracellular expression of Frataxin / FXN within hepatic-derived cells. The observed staining profile is consistent with detection of this mitochondrial iron metabolism protein involved in iron-sulfur cluster assembly and oxidative phosphorylation-associated cellular pathways. Blue=FXN antibody, Green=isotype control, Red=blank control.