FUT1 Antibody / Blood Group H Antigen Enzyme Antibody (R31996)

FUT1 Antibody WB. Western blot testing of 1) rat brain, 2) human SW620 and 3) A549 lysate with FUT1 antibody. Predicted/observed molecular weight ~50 kDa.

FUT1 Antibody Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue using FUT1 Antibody demonstrates moderate to strong membranous and cytoplasmic HRP-DAB brown staining within malignant epithelial cells lining glandular and duct-like tumor structures. The staining pattern is consistent with expression of Fucosyltransferase 1 (FUT1), a Golgi-associated glycosyltransferase responsible for blood group H antigen biosynthesis and regulation of cell surface fucosylation. FUT1-mediated glycosylation influences cellular adhesion, receptor function, and cell-cell recognition processes, and altered expression has been associated with cancer-related changes in glycan composition and tumor cell behavior. The observed staining highlights neoplastic epithelial populations and is consistent with the known role of FUT1 in regulating biologically active cell surface carbohydrate structures. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

FUT1 Antibody Mouse Intestine IHC. Immunohistochemistry staining of FFPE mouse intestine using FUT1 Antibody demonstrates prominent membranous and apical cytoplasmic HRP-DAB brown staining within intestinal epithelial cells lining the mucosal villi. The staining pattern is consistent with expression of Fucosyltransferase 1 (FUT1), a glycosyltransferase responsible for synthesis of blood group H antigen and generation of fucosylated cell surface glycans. Strong epithelial localization is consistent with the established role of FUT1 in gastrointestinal tissues, where fucosylation contributes to epithelial differentiation, mucosal barrier function, host-microbe interactions, and regulation of cell surface carbohydrate antigens. The observed staining highlights the apical epithelial compartment, a major site of glycan biosynthesis and carbohydrate antigen expression within the intestinal mucosa. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

FUT1 Antibody Rat Intestine IHC. Immunohistochemistry staining of FFPE rat intestine using FUT1 Antibody demonstrates distinct membranous and apical cytoplasmic HRP-DAB brown staining throughout the intestinal epithelial lining. The staining pattern is consistent with expression of Fucosyltransferase 1 (FUT1), an alpha(1,2)-fucosyltransferase responsible for blood group H antigen biosynthesis and generation of fucosylated glycan structures on the cell surface. Intestinal epithelial localization is consistent with the known role of FUT1 in regulating mucosal glycosylation, epithelial differentiation, host-microbe interactions, and maintenance of gastrointestinal barrier function. The prominent staining observed along the luminal epithelial surface highlights tissues actively engaged in glycan biosynthesis and carbohydrate antigen expression. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.