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Home >> Antibodies >> FUBP2 Antibody / Far upstream element-binding protein 2 / KHSRP

FUBP2 Antibody / Far upstream element-binding protein 2 / KHSRP (RQ7979)

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  Catalog No Formulation Size Price (USD)  
Image RQ7979 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) rat stomach, 3) mouse brain and 4) mouse stomach tissue lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
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Western blot testing of 1) rat brain, 2) rat stomach, 3) mouse brain and 4) mouse stomach tissue lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
Western blot testing of human 1) HEK293, 2) HeLa, 3) MCF7, 4) Jurkat, 5) K562, 6) Caco-2 and 7) U-937 cell lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
Immunofluorescent staining of FFPE human Caco-2 cells with FUBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gallbladder adenocarcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human mouse brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human K562 cells with FUBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FUBP2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q92945
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This FUBP2 antibody is available for research use only.
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Description

Far upstream element-binding protein 2 is a protein that in humans is encoded by the KHSRP gene. The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization.

Application Notes

Optimal dilution of the FUBP2 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids D116-R478) was used as the immunogen for the FUBP2 antibody.

Storage

After reconstitution, the FUBP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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