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Home >> Antibodies >> FUBP2 Antibody / Far upstream element-binding protein 2 / KHSRP

FUBP2 Antibody / Far upstream element-binding protein 2 / KHSRP (RQ7979)

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  Catalog No Formulation Size Price (USD)  
Image RQ7979 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Western blot testing of 1) rat brain, 2) rat stomach, 3) mouse brain and 4) mouse stomach tissue lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
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Western blot testing of 1) rat brain, 2) rat stomach, 3) mouse brain and 4) mouse stomach tissue lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
Western blot testing of human 1) HEK293, 2) HeLa, 3) MCF7, 4) Jurkat, 5) K562, 6) Caco-2 and 7) U-937 cell lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
Immunofluorescent staining of FFPE human Caco-2 cells with FUBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gallbladder adenocarcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human mouse brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human fixed and permeabilized K562 cells with FUBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FUBP2 antibody.
Immunoprecipitation of FUBP2 protein from 500ug of human K562 whole cell lysate with 2ug of FUBP2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q92945
Localization Cytoplasmic, nuclear
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Immunoprecipitation : 2ug per 500ug of lysate
Direct ELISA : 0.1-0.5ug/ml
Limitations This FUBP2 antibody is available for research use only.
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Description

Far upstream element-binding protein 2 (FUBP2), also known as KHSRP, is an RNA-binding protein that regulates mRNA stability, splicing, and decay. It plays an essential role in post-transcriptional gene regulation by binding AU-rich elements in the 3′ untranslated regions of target mRNAs, influencing their turnover and translation.

FUBP2 is involved in multiple cellular processes, including cell differentiation, immune responses, and neuronal development. It also participates in microRNA biogenesis and contributes to fine-tuning gene expression networks. Altered FUBP2 function has been linked to immune dysregulation, cancer progression, and neurological disorders, underscoring its biomedical importance.

Using a high-quality FUBP2 antibody enables precise detection in applications such as western blot, immunohistochemistry, and RNA-binding protein studies. A FUBP2 antibody from NSJ Bioreagents ensures reproducibility and sensitivity for investigating post-transcriptional regulation, mRNA decay, and disease mechanisms. Selecting the right FUBP2 antibody is key to generating accurate and consistent results.

Application Notes

Optimal dilution of the FUBP2 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids D116-R478) was used as the immunogen for the FUBP2 antibody.

Storage

After reconstitution, the FUBP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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