FOXP3 Antibody (R30134)

Description

FOXP3 is a member of the forkhead box family of transcription factors and is considered a master regulator of regulatory T cell development and function. It is highly expressed in CD4 positive CD25 positive regulatory T cells, where it plays a critical role in maintaining immune tolerance and preventing autoimmune responses. The importance of FOXP3 is underscored by its association with immune dysregulation syndromes when mutated or dysregulated.

The FOXP3 gene undergoes alternative splicing, resulting in multiple isoforms that may differ in their regulatory functions and cellular localization. The full-length isoform is the most widely studied, but shorter isoforms lacking specific domains have also been identified and may modulate transcriptional activity or interact with co-regulators differently.

A FOXP3 antibody is an essential tool for immunologists studying regulatory T cells and immune regulation. Researchers rely on a FOXP3 antibody for applications such as immunohistochemistry, flow cytometry, western blot, and immunofluorescence to assess expression levels, localization, and functional states of T cells. NSJ Bioreagents provides a high-quality FOXP3 antibody that ensures reliable detection across various sample types and assay conditions. With rigorous validation and performance data, the FOXP3 antibody from NSJ Bioreagents supports accurate and reproducible results for both basic research and clinical investigations.

Application Notes

The stated application concentrations are suggested starting points. Titration of the FOXP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 101-431) was used as the immunogen for this FOXP3 antibody.

Storage

After reconstitution, the FOXP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.