- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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FOXO3A Antibody / Forkhead Transcription Factor Antibody recognizes FOXO3A, a member of the Forkhead box O family of transcription factors that regulates gene expression controlling apoptosis, cell cycle arrest, oxidative stress resistance, metabolism, autophagy, DNA repair, and longevity. FOXO3A functions as a sequence-specific DNA-binding protein that integrates signals from multiple intracellular pathways to determine whether cells proliferate, differentiate, survive, or undergo programmed cell death. As one of the best-characterized forkhead transcription factors, FOXO3A serves as a key downstream effector of the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway and numerous stress-responsive signaling cascades. An FOXO3A Antibody is therefore widely used to investigate transcriptional regulation, signal transduction, and cellular stress responses.
FOXO3A activity is tightly regulated through phosphorylation, acetylation, ubiquitination, and additional post-translational modifications that influence its intracellular localization and transcriptional activity. Under growth factor stimulation, AKT phosphorylates FOXO3A, promoting its association with 14-3-3 proteins and export from the nucleus, thereby suppressing transcription of genes involved in apoptosis and stress resistance. In contrast, oxidative stress, nutrient deprivation, and other cellular challenges promote FOXO3A nuclear accumulation and activation of protective transcriptional programs. Researchers frequently analyze FOXO3A together with AKT, mTOR, PTEN, and additional signaling molecules to better understand the balance between cell survival and programmed cell death. An FOXO3A Antibody provides an important tool for investigating these highly regulated signaling events and the broader biology of forkhead transcription factors.
Altered FOXO3A expression or activity has been implicated in numerous human diseases, including breast, prostate, lung, colorectal, ovarian, and hematologic cancers, where dysregulated PI3K/AKT signaling often suppresses FOXO3A tumor suppressor function. FOXO3A has also been linked to cardiovascular disease, diabetes, neurodegenerative disorders, skeletal muscle homeostasis, immune regulation, stem cell maintenance, and healthy aging. Genetic studies have associated FOXO3A variants with exceptional longevity in multiple human populations, highlighting its broad physiological importance. Consequently, an FOXO3A Antibody has become an essential reagent for research spanning oncology, metabolism, neuroscience, immunology, aging, and signal transduction.
FOXO3A is routinely analyzed using Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, chromatin immunoprecipitation, and flow cytometry to evaluate protein expression, nuclear localization, transcriptional activity, and regulation by upstream signaling pathways. When paired with phospho-specific antibodies, investigators can distinguish changes in total FOXO3A abundance from phosphorylation-dependent regulation. NSJ Bioreagents provides carefully validated antibodies to support these diverse applications across multiple experimental models. An FOXO3A Antibody / Forkhead Transcription Factor Antibody provides researchers with a reliable reagent for investigating transcriptional regulation, apoptosis, oxidative stress responses, cell cycle control, and disease-associated signaling mechanisms.
Explore our Transcription Factor Antibodies page to discover validated antibodies for FOXO3A and other transcriptional regulators involved in apoptosis, oxidative stress responses, PI3K/AKT signaling, cell cycle control, and gene expression.
Optimal dilution of the FOXO3A Antibody / Forkhead Transcription Factor Antibody should be determined by the researcher.
A synthetic peptide specific to human FOXO3A was used as the immunogen for the FOXO3A antibody.
Store the FOXO3A antibody at -20oC.
FOXO3A antibody, Forkhead Transcription Factor antibody, Forkhead Box O3 antibody, FKHRL1 antibody
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