- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Forkhead Box O3 Antibody / FOXO3A Antibody recognizes Forkhead Box O3 (FOXO3A), a member of the Forkhead box O family of transcription factors that coordinates gene expression programs controlling apoptosis, oxidative stress resistance, autophagy, metabolism, DNA repair, and cell cycle progression. The designation Forkhead Box O3 emphasizes the protein's membership within the highly conserved forkhead transcription factor family, whose members regulate diverse physiological processes through sequence-specific DNA binding. Because FOXO3A integrates signals from growth factors, nutrient availability, and cellular stress, a Forkhead Box O3 Antibody is widely used to investigate transcriptional regulation, intracellular signaling, and mechanisms that maintain cellular homeostasis.
Forkhead Box O3 functions downstream of several major signaling pathways, most notably the phosphoinositide 3-kinase (PI3K)/AKT cascade. Under growth-promoting conditions, AKT phosphorylates FOXO3A, resulting in its association with 14-3-3 proteins, export from the nucleus, and inhibition of transcriptional activity. During oxidative stress or nutrient deprivation, FOXO3A translocates back to the nucleus where it activates genes involved in antioxidant defense, apoptosis, autophagy, DNA damage repair, and metabolic adaptation. Researchers frequently analyze Forkhead Box O3 alongside AKT, mTOR, PTEN, and additional signaling proteins to better understand the dynamic regulation of cell survival and stress responses. A Forkhead Box O3 Antibody therefore serves as an important reagent for studies of transcription factor biology and signal transduction.
Abnormal regulation of Forkhead Box O3 has been implicated in numerous diseases, including breast, prostate, lung, colorectal, ovarian, and hematologic cancers, where suppression of FOXO3A activity contributes to tumor progression and therapeutic resistance. Beyond oncology, FOXO3A has been associated with diabetes, cardiovascular disease, neurodegenerative disorders, immune regulation, skeletal muscle maintenance, stem cell biology, and healthy aging. Human genetic studies have further identified FOXO3A variants associated with exceptional longevity, underscoring its importance in maintaining long-term cellular function. Consequently, a Forkhead Box O3 Antibody has become an essential research tool for investigations spanning cancer biology, metabolism, neuroscience, immunology, and aging research.
Forkhead Box O3 is routinely analyzed using Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, chromatin immunoprecipitation, and flow cytometry to evaluate protein expression, intracellular localization, and transcriptional regulation. When used together with phospho-specific FOXO3A antibodies, researchers can distinguish changes in total protein abundance from phosphorylation-dependent modulation of activity. NSJ Bioreagents provides carefully validated antibodies to support these applications across diverse experimental systems. A Forkhead Box O3 Antibody / FOXO3A Antibody provides researchers with a dependable reagent for investigating transcriptional regulation, apoptosis, oxidative stress responses, PI3K/AKT signaling, and mechanisms governing cellular fate.
Visit our FOXO3A Antibody / Forkhead Transcription Factor Antibody page for additional information about validated antibodies for investigating FOXO3A expression, forkhead transcription factor biology, PI3K/AKT signaling, apoptosis, and oxidative stress responses.
Optimal dilution of the Forkhead Box O3 Antibody / FOXO3A Antibody should be determined by the researcher.
A synthetic peptide specific to human FOXO3A was used as the immunogen for the FOXO3A antibody.
Store the FOXO3A antibody at -20oC.
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