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Home >> Antibodies >> FOXO1 Antibody / Forkhead box protein O1

FOXO1 Antibody / Forkhead box protein O1 [clone FOXO1/9792] (V5702)

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  Catalog No Formulation Size Price (USD)  
Image V5702-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5702-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5702SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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Western blot testing of human brain tissue lysate with FOXO1 antibody (clone FOXO1/9792). Predicted molecular weight ~70 kDa.
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Western blot testing of human brain tissue lysate with FOXO1 antibody (clone FOXO1/9792). Predicted molecular weight ~70 kDa.
IHC staining of FFPE human brain tissue with FOXO1 antibody (clone FOXO1/9792). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free FOXO1 antibody (clone FOXO1/9792) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name FOXO1/9792
Purity Protein G affinity
UniProt Q12778
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This FOXO1 antibody is available for research use only.
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Description

FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxyterminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes.

Application Notes

Optimal dilution of the FOXO1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 450-650 from human FOXO1 protein was used as the immunogen for the FOXO1 antibody.

Storage

Aliquot the FOXO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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