FOXI1 Antibody / Forkhead Box Transcription Factor Marker [clone PCRP-FOXI1-1F8] (V9554)

FOXI1 Antibody HeLa IF. Immunofluorescence analysis of PFA-fixed human HeLa cells stained with FOXI1 Antibody / Forkhead Box Transcription Factor Marker (green, clone PCRP-FOXI1-1F8) and phalloidin cytoskeletal stain (red). Forkhead box protein I1 / FOXI1 demonstrates prominent nuclear staining with additional cytoplasmic signal in human HeLa cells, consistent with the intracellular distribution expected for a forkhead family transcription-associated regulatory protein involved in epithelial differentiation and developmental gene expression pathways.

FOXI1 Antibody Prostate IHC. Immunohistochemistry analysis of FFPE human prostate tissue stained with FOXI1 Antibody / Forkhead Box Transcription Factor Marker (clone PCRP-FOXI1-1F8). Nuclear HRP-DAB brown staining is observed in prostate epithelial cells, consistent with the transcription-associated localization expected for Forkhead box protein I1 / FOXI1. Sections underwent HIER using 10mM Tris with 1mM EDTA buffer, pH 9, followed by incubation with FOXI1 antibody at 2ug/ml prior to chromogenic detection.

FOXI1 Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells stained with FOXI1 Antibody / Forkhead Box Transcription Factor Marker (blue, clone PCRP-FOXI1-1F8). Unstained cells are shown in red. The distinct fluorescence shift supports detection of endogenous Forkhead box protein I1 / FOXI1 expression in HeLa cells, consistent with the broad intracellular distribution expected for a forkhead family transcription-associated regulatory protein involved in epithelial differentiation and developmental signaling pathways.

SDS-PAGE analysis of purified, BSA-free FOXI1 antibody (clone PCRP-FOXI1-1F8) as confirmation of integrity and purity.

FOXI1 Antibody HuProt Microarray Specificity Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using FOXI1 Antibody / Forkhead Box Transcription Factor Marker clone PCRP-FOXI1-1F8 demonstrates exceptional specificity for Forkhead box protein I1 / FOXI1. FOXI1 ranks first among all proteins analyzed on the array, with extremely strong separation from secondary signals, supporting the highly selective recognition profile of this mouse monoclonal antibody for forkhead transcription factor and developmental signaling research applications. Z-score represents signal intensity above background in standard deviation units, while S-score reflects the relative specificity of antibody binding compared with other proteins present on the HuProt(TM) platform.