FOXD1 Antibody / Developmental Transcription Factor Antibody (F55106)

FOXD1 Antibody Human and Mouse WB. Western blot analysis of human HEK293, U-2 OS, U-87 MG, and Y79 cell lysates, as well as mouse kidney and mouse eyeball tissue lysates, using FOXD1 antibody demonstrates detection of a prominent immunoreactive band at approximately 46-55 kDa, consistent with expression of Forkhead Box Protein D1 (FOXD1). FOXD1 is a developmental transcription factor that regulates gene expression programs involved in tissue patterning, cellular differentiation, and organogenesis. The observed expression across both cultured cell lines and mouse tissues is consistent with the role of FOXD1 in developmental regulatory networks and maintenance of tissue-specific gene expression programs. Predicted molecular weight: ~46 kDa.

FOXD1 Antibody Human Kidney IHC. Immunohistochemistry staining of FFPE human kidney tissue using FOXD1 antibody demonstrates prominent nuclear staining within renal tubular epithelial cells, consistent with expression of Forkhead Box Protein D1 (FOXD1). FOXD1 is a developmental transcription factor that regulates gene expression programs involved in tissue patterning, cellular differentiation, and maintenance of specialized cellular identities. The observed nuclear localization is consistent with the role of FOXD1 as a DNA-binding transcriptional regulator and supports its involvement in developmental and tissue-specific regulatory pathways within the kidney. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

FOXD1 Antibody HepG2 Cell IF. Immunofluorescent staining of fixed and permeabilized human HepG2 cells using FOXD1 antibody demonstrates strong nuclear localization of Forkhead Box Protein D1 (FOXD1), shown in green. FOXD1 is a developmental transcription factor that regulates gene expression programs involved in cellular differentiation, tissue patterning, and organogenesis. The observed nuclear staining pattern is consistent with the role of FOXD1 as a DNA-binding transcriptional regulator controlling developmental and tissue-specific gene expression networks. Phalloidin staining (red) highlights the actin cytoskeleton and provides contrast to the predominantly nuclear distribution of FOXD1. These findings support the utility of FOXD1 antibody for studies of developmental biology, transcriptional regulation, and cellular differentiation pathways.