FOXC1 Antibody / Forkhead Transcription Factor Antibody (RQ5578)

FOXC1 Antibody WB. Western blot analysis of human HEK293 cell lysate using FOXC1 Antibody / Forkhead Transcription Factor Antibody demonstrates a prominent immunoreactive band at approximately 70-75 kDa, consistent with expression of FOXC1. Although the predicted molecular weight of FOXC1 is approximately 57 kDa, the protein is frequently observed at higher apparent molecular weights due to post-translational modifications, including phosphorylation and other regulatory modifications that influence electrophoretic mobility. FOXC1 is a forkhead box transcription factor that regulates gene expression programs involved in embryonic development, tissue patterning, cellular differentiation, and stem cell-associated signaling pathways. The observed banding pattern is consistent with endogenous FOXC1 expression in human cells. Predicted molecular weight: approximately 57 kDa. Commonly observed molecular weight: up to 75 kDa.

FOXC1 Antibody 293T Cell WB. Western blot analysis of human 293T cell lysate using FOXC1 Antibody / Forkhead Transcription Factor Antibody demonstrates a distinct immunoreactive band at approximately 70-75 kDa, consistent with expression of FOXC1. Although the predicted molecular weight of FOXC1 is approximately 57 kDa, the protein is frequently observed at higher apparent molecular weights due to post-translational modifications that affect electrophoretic mobility. FOXC1 is a forkhead box transcription factor that regulates gene expression programs involved in embryonic development, tissue patterning, cellular differentiation, stem cell maintenance, and organogenesis. The observed banding pattern is consistent with endogenous FOXC1 expression in human 293T cells. Predicted molecular weight: approximately 57 kDa. Commonly observed molecular weight: up to 75 kDa.

FOXC1 Antibody IF. Immunofluorescent staining of FFPE human HeLa cells using FOXC1 Antibody / Forkhead Transcription Factor Antibody demonstrates green fluorescence localized predominantly within cell nuclei, consistent with the expected localization of FOXC1 as a DNA-binding transcription factor. FOXC1 regulates gene expression programs involved in embryonic development, cellular differentiation, tissue patterning, and stem cell-associated signaling pathways. Co-staining with Alpha Tubulin monoclonal antibody (red) highlights the cytoskeletal network and provides cellular context for nuclear FOXC1 expression. HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunofluorescent staining. Green = FOXC1 antibody; Red = Alpha Tubulin monoclonal antibody; nuclei appear green due to nuclear FOXC1 localization.