FOXA1 Antibody (RQ4009)

FOXA1 Antibody MCF-7 Cell FACS. Flow cytometry analysis of human MCF-7 cells using FOXA1 Antibody demonstrates a pronounced rightward shift of the FOXA1-positive population (blue) relative to the isotype control (green) and unstained cells (red), consistent with expression of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that controls developmental gene expression, epithelial differentiation, chromatin accessibility, and tissue-specific transcriptional programs. As a developmental transcription factor, FOXA1 plays a central role in establishing cellular identity and maintaining differentiated epithelial phenotypes through regulation of lineage-specific gene expression networks. Red=cells alone; Green=isotype control; Blue=FOXA1 antibody.

FOXA1 Antibody Multi-Cell Line Western Blot. Western blot analysis of human MCF7 (lane 1), HepG2 (lane 2), and RT4 (lane 3) cell lysates using FOXA1 Antibody demonstrates distinct bands at approximately 49 kDa, consistent with the predicted molecular weight of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that facilitates chromatin accessibility and controls developmental gene expression programs involved in organogenesis, epithelial differentiation, and tissue-specific cellular identity. Detection of the expected molecular weight band across multiple epithelial-derived cell lines supports expression of FOXA1 and its role as a developmental transcription factor that coordinates lineage-specific transcriptional networks and differentiated cellular phenotypes.