FOXA1 Antibody [clone FHBA1-1] (V7225)

FOXA1 Antibody Human Bladder Carcinoma IHC. Immunohistochemical staining of FFPE human bladder carcinoma tissue using FOXA1 Antibody (clone FHBA1-1) demonstrates strong nuclear HRP-DAB brown staining throughout malignant epithelial cell populations. The staining pattern is consistent with expression of FOXA1, a forkhead family transcription factor that functions as a developmental regulator of gene expression, epithelial differentiation, and tissue-specific transcriptional programs. The predominantly nuclear localization is characteristic of a DNA-binding transcription factor and supports the role of FOXA1 in maintaining epithelial cellular identity and regulating lineage-specific gene expression networks. Staining of FFPE tissue is enhanced by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

FOXA1 Antibody Human Prostate Carcinoma IHC. Immunohistochemical staining of FFPE human prostate carcinoma tissue using FOXA1 Antibody (clone FHBA1-1) demonstrates strong nuclear HRP-DAB brown staining within malignant epithelial cell populations. The staining pattern is consistent with expression of FOXA1, a forkhead family transcription factor that functions as a developmental regulator of gene expression, epithelial differentiation, and tissue-specific transcriptional programs. The prominent nuclear localization is characteristic of a DNA-binding transcription factor and supports the role of FOXA1 in maintaining epithelial lineage identity and regulating gene networks associated with cellular differentiation and tissue development. Staining of FFPE tissue is enhanced by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

FOXA1 Antibody MCF7 Cell IF. Immunofluorescent staining of PFA-fixed human MCF7 cells using FOXA1 Antibody (clone FHBA1-1) demonstrates strong green nuclear staining consistent with localization of FOXA1, a forkhead family transcription factor that regulates developmental gene expression, epithelial differentiation, and tissue-specific transcriptional programs. The predominantly nuclear staining pattern is characteristic of a DNA-binding transcriptional regulator and supports the role of FOXA1 in controlling gene networks that govern cellular identity, lineage specification, and differentiated epithelial phenotypes. Phalloidin (red) labels the actin cytoskeleton and outlines cellular morphology, providing structural contrast to the nuclear localization of FOXA1.

FOXA1 Antibody MCF-7 Cell FACS. Flow cytometry analysis of PFA-fixed human MCF-7 cells using FOXA1 Antibody (clone FHBA1-1) demonstrates a distinct rightward shift of the FOXA1-positive population (blue) relative to the isotype control (red), consistent with expression of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that controls developmental gene expression, epithelial differentiation, chromatin accessibility, and tissue-specific transcriptional programs. As a developmental transcription factor, FOXA1 plays a central role in establishing cellular identity and maintaining differentiated epithelial phenotypes through regulation of lineage-specific gene expression networks. Red=isotype control; Blue=FOXA1 antibody.

FOXA1 Antibody HeLa Cell WB. Western blot analysis of human HeLa cell lysate using FOXA1 Antibody (clone FHBA1-1) demonstrates a distinct band at approximately 49 kDa, consistent with the predicted molecular weight of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that facilitates chromatin accessibility and controls developmental gene expression programs involved in organogenesis, epithelial differentiation, and tissue-specific cellular identity. Detection of the expected molecular weight band supports expression of FOXA1 and its role as a developmental transcription factor that coordinates lineage-specific transcriptional networks and differentiated cellular phenotypes.