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Home >> Antibodies >> Fox2 Antibody / RBM9

Fox2 Antibody / RBM9 (RQ5548)

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  Catalog No Formulation Size Price (USD)  
Image RQ5548 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) U-2 OS, 2) Caco-2, 3) A431, 4) U-87 MG, 5) rat brain and 6) mouse brain lysate with Fox2 antibody. Expected molecular weight ~41 kDa.
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Western blot testing of human 1) U-2 OS, 2) Caco-2, 3) A431, 4) U-87 MG, 5) rat brain and 6) mouse brain lysate with Fox2 antibody. Expected molecular weight ~41 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with Fox2 antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human SiHa cells with Fox2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fox2 antibody.
IHC staining of FFPE human melanoma with Fox2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse gastric tissue with Fox2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat gastric tissue with Fox2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat gastric tissue with Fox2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O43251
Localization Nuclear
Applications Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Fox2 antibody is available for research use only.
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Description

RNA binding motif protein 9 (RBM9), also known as Rbfox2, is a protein which in humans is encoded by the RBM9 gene. It is mapped to 22q12.3. This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Fox2 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids M1-Q81) was used as the immunogen for the Fox2 antibody.

Storage

After reconstitution, the Fox2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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