Folate receptor 1 Antibody / FOLR1 [clone FOLR1/12903R] (V6153)

Western blot analysis of Folate receptor alpha expression in tissue and cell lysates. Lysates from human brain, mouse brain, rat brain, and human HeLa cells were analyzed by western blot using recombinant Folate receptor 1 antibody (clone FOLR1/12903R). A single immunoreactive band is detected in human brain and HeLa lysates, while little to no signal is observed in mouse and rat brain samples. Although the predicted molecular weight of Folate receptor alpha is approximately 30 kDa based on sequence analysis, the protein is extensively glycosylated, resulting in a higher apparent molecular weight on SDS-PAGE.

Western blot analysis of Folate receptor alpha expression in human brain tissue. Lysates from human brain was analyzed by western blot using recombinant Folate receptor 1 antibody (clone FOLR1/12903R). A single immunoreactive band is detected in human brain lysate. Although the predicted molecular weight of Folate receptor alpha is approximately 30 kDa based on sequence analysis, the protein is extensively glycosylated, resulting in a higher apparent molecular weight on SDS-PAGE.

Immunohistochemistry of Folate receptor alpha in human adrenal gland tissue. Formalin-fixed, paraffin-embedded human adrenal gland tissue stained with recombinant Folate receptor 1 antibody (clone FOLR1/12903R) shows cytoplasmic punctate staining in adrenal cortical cells, with minimal signal in surrounding stromal regions. Inset shows negative control with PBS substituted for the primary antibody. Antigen retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, at 95oC for 45 minutes, followed by cooling at room temperature for 20 minutes.