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Home >> Antibodies >> Focal Adhesion Kinase Antibody / FAK1 / PTK2

Focal Adhesion Kinase Antibody / FAK1 / PTK2 (F54458)

  Catalog No Formulation Size Price (USD)  
Image F54458-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54458-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human T47-D cell lysate with Focal Adhesion Kinase antibody. Predicted molecular weight ~119 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Focal Adhesion Kinase antibody.
Immunofluorescent staining of human HeLa cells with Focal Adhesion Kinase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human brain tissue with Focal Adhesion Kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with Focal Adhesion Kinase antibody; Blue=isotype control, Green= Focal Adhesion Kinase antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity SAS precipitation
UniProt Q05397
Applications Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This Focal Adhesion Kinase antibody is available for research use only.
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Description

FAK1/PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Focal Adhesion Kinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 396-423 from the human protein was used as the immunogen for the Focal Adhesion Kinase antibody.

Storage

Aliquot the Focal Adhesion Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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