FN1 Antibody / Microarray Specificity Validated [clone FN1/2948] (V8387)

FN1 Antibody Human Lung IHC. Immunohistochemistry analysis of FFPE human lung tissue stained with clone FN1/2948. Extracellular and stromal HRP-DAB brown staining is observed throughout connective tissue and alveolar-associated matrix regions, consistent with expression of Fibronectin 1 / FN1 within extracellular matrix remodeling and tissue organization pathways. Clone FN1/2948 is supported by HuProt(TM) protein microarray specificity validation demonstrating highly selective recognition of FN1 in complex protein environments. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FN1 Antibody Lung Stromal Matrix IHC. Immunohistochemistry analysis of FFPE human lung tissue stained with clone FN1/2948. Diffuse extracellular HRP-DAB brown staining highlights stromal connective tissue and matrix-associated structures consistent with expression of Fibronectin 1 / FN1, a major extracellular matrix glycoprotein involved in tissue remodeling and cellular adhesion pathways. Clone FN1/2948 is supported by HuProt(TM) protein microarray specificity validation demonstrating selective recognition of FN1 in complex extracellular matrix environments. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FN1 Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone FN1/2948. These results demonstrate high specificity of the mouse monoclonal antibody for Fibronectin 1 / FN1, a major extracellular matrix glycoprotein involved in stromal organization, tissue remodeling, and integrin-mediated adhesion pathways. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array, supporting the microarray specificity validated differentiation of clone FN1/2948. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.

SDS-PAGE analysis of purified, BSA-free FN1 antibody (clone FN1/2949) as confirmation of integrity and purity.