FMO4 Antibody / Flavin-Containing Monooxygenase 4 Polyclonal Antibody (R30983)

FMO4 Antibody by IF in human MCF-7 cells. Immunofluorescence staining of human MCF-7 cells using FMO4 Antibody at 5 ug/ml. Green fluorescence indicates FMO4 immunoreactivity, while DAPI counterstaining identifies nuclei in blue. The image shows predominantly extranuclear green staining distributed throughout the cytoplasm, with areas of granular and reticular fluorescence and some enhancement near cell boundaries. Nuclear regions show comparatively little green signal. The cytoplasmic distribution is compatible with the microsomal localization of this enzyme, although the image does not independently establish association with a particular organelle. Enzymatic antigen retrieval was performed before overnight primary antibody incubation at 4oC. Detection used a Fluoro488-conjugated secondary antibody. This Flavin-Containing Monooxygenase 4 Polyclonal Antibody demonstrates cellular immunoreactivity.

FMO4 Antibody by WB in human cell lines and rodent tissues. Western blot analysis using FMO4 Antibody on samples separated by 10% SDS-PAGE under reducing conditions, with 30 ug of protein loaded per lane. Lane 1: human THP-1 cells; lane 2: human U251 cells; lane 3: human Jurkat cells; lane 4: human MCF-7 cells; lane 5: rat liver; lane 6: mouse kidney; lane 7: mouse liver. A prominent band appears near 63 kDa in all seven lanes, consistent with the predicted molecular weight of approximately 63.3 kDa. Some samples show additional weaker bands near the primary signal, particularly U251 and MCF-7. The Flavin-Containing Monooxygenase 4 Polyclonal Antibody was used at 0.5 ug/ml.