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Home >> Antibodies >> Flt3 Antibody / CD135

Flt3 Antibody / CD135 (R32824)

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  Catalog No Formulation Size Price (USD)  
Image R32824 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of mouse brain lysate with Flt3 antibody at 0.5ug/ml. Predicted molecular weight: ~113 kDa (unmodified), ~130/160 kDa (glycosylated).
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Western blot testing of mouse brain lysate with Flt3 antibody at 0.5ug/ml. Predicted molecular weight: ~113 kDa (unmodified), ~130/160 kDa (glycosylated).
IHC testing of FFPE mouse small intestine tissue with Flt3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse kidney tissue with Flt3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat kidney tissue with Flt3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with Flt3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat liver tissue with Flt3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q00342
Localization Cell membrane, cytoplasm
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA (mouse recombinant protein) : 0.1-0.5ug/ml (BSA-free formulation available)
Limitations This Flt3 antibody is available for research use only.
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Description

Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow.

Application Notes

Optimal dilution of the Flt3 antibody should be determined by the researcher.

Immunogen

A recombinant mouse protein corresponding to amino acids E62-E295 was used as the immunogen for the Flt3 antibody.

Storage

After reconstitution, the Flt3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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