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Home >> Antibodies >> FLAG Antibody

FLAG Antibody (F52063)

  Catalog No Formulation Size Price (USD)  
Image F52063-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F52063-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot analysis of lysate from a 41 kDa 12 tagged protein using FLAG antibody at 1:1000.
Availability 1-3 business days
Species Reactivity DYKDDDDK
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
Applications Western blot : 1:1000
Limitations This FLAG antibody is available for research use only.
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Description

Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion.

Application Notes

Titration of the FLAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

The sequence cDYKDDDDK was used as the immunogen for this FLAG antibody.

Storage

Aliquot the FLAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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