Fibronectin Antibody [clone SPM539] (V9020)

Description

Fibronectin is a dimeric glycoprotein of 440kDa which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This mAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin.

Researchers investigating extracellular matrix remodeling, stromal organization, and integrin-mediated adhesion pathways may also be interested in our Fibronectin 1 Antibody / Extracellular Matrix Marker page featuring validated immunohistochemistry, western blot, and protein microarray specificity data for ECM biology research.

Application Notes

The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

Purified human fibronectin was used as the immunogen for this Fibronectin antibody.

Storage

Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).