Fibronectin 1 Antibody / Extracellular Matrix Marker [clone FN1/3569] (V9460)

Fibronectin 1 Antibody Human Lung IHC. Immunohistochemistry analysis of FFPE human lung tissue stained with clone FN1/3569. Extracellular and pericellular HRP-DAB brown staining is observed within stromal connective tissue and alveolar-associated matrix structures, consistent with expression of Fibronectin 1 / FN1, a major extracellular matrix glycoprotein involved in tissue remodeling, cellular adhesion, and stromal organization pathways. Surrounding epithelial cells show comparatively lower staining intensity. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Fibronectin 1 Antibody Lung Connective Tissue IHC. Immunohistochemistry analysis of FFPE human lung tissue stained with clone FN1/3569 at 2 ug/ml in PBS for 30 min at RT. Strong extracellular HRP-DAB brown staining highlights stromal connective tissue and matrix-rich peribronchiolar regions consistent with expression of Fibronectin 1 / FN1, an extracellular matrix glycoprotein involved in tissue remodeling, integrin-mediated adhesion, and structural organization of the pulmonary microenvironment. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Fibronectin 1 Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone FN1/3569. These results demonstrate high specificity of the mouse monoclonal antibody for Fibronectin 1 / FN1, a major extracellular matrix glycoprotein involved in tissue remodeling, stromal organization, and integrin-mediated adhesion pathways. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.

Fibronectin 1 Antibody Placenta WB. Western blot analysis of human placenta tissue lysate using Fibronectin 1 Antibody / Extracellular Matrix Marker. A strong high molecular weight band is detected at approximately 220-250 kDa, consistent with the expected molecular weight range of Fibronectin 1 / FN1. The broad signal pattern is characteristic of this heavily glycosylated extracellular matrix glycoprotein and may reflect glycosylated and dimeric fibronectin species associated with stromal matrix organization and tissue remodeling pathways.